FLU-LAB-NET video resources

Influenza-detection of Influenza A matrix gene by real time RT-PCR video of a standard operating procedure

Disclaimer

These videos provide advice and outline basic practices and are not designed as a stand alone training tool.

The videos target regulations that were up to date at the date of filming in July 2010.  The procedures shown relate to local regulations specific to the UK and the Animal Health & Veterinary Laboratories Agency AI Community Reference Laboratory.  Some of the practices have been modified for filming purposes.  Information in these films should not be used without checking information from appropriate, up to date, sources and obtaining professional advice where it is prudent to do so. 

Although the videos mention Virkon S as the disinfectant used, there are many other approved disinfectants available.

Glossary

Notifiable disease notifiable disease is any disease that is required by law to be reported to government authorities. The collation of information allows the authorities to monitor the disease, and provides early warning of possible outbreaks. Many governments have regulations for reporting of both human and animal (generally livestock) diseases.

ACDP  The Advisory Committee on Dangerous Pathogens (ACDP) is a UK-wide non-departmental advisory public body.  They advise UK ministries on all aspects of hazards and risks to workers and others from exposure to pathogens".

BSL 3+  As, BSL3 but with some additional safety features including double HEPA filtered extract air, shower on exit and double ended autoclave which enables work with SAPO 4 organisms.

SAPO Specified Animal Pathogens Order (UK legislation on the handling of specified animal pathogens).

HEPA filter  A High Efficiency Particulate Air filter

Building being ‘taken down’:  The VLA uses the term ‘taken down’ to describe when a High Security Biocontainment facility has been full decontaminated and fumigated and can be opened up to engineers etc for maintenance when no high containment work is being completed.  

Waste streams  The ways in which waste materials exit the building, whether by fumigation or disinfection and then autoclaving or by decontamination. 

‘Approved disinfectants’   In the UK there is a statutory requirement for cleansing and disinfection and only approved disinfectants can be used in the event of an outbreak and these must be approved by Defra. 

 Supplementary: Tables from WHO Laboratory Biosafety Manual.  Third Edition